MikeDemakes
New member
Hey, guys. I did a lab last week where we had to determine the nicotine in tobacco and I am having trouble with the calculations.
It was determined that the calculated molarity of HCLO4 was 0.03835M.
3.0117 grams of tobacco was weighed out and shaken vigorously when 100 mL of 90% toluene-10% chloroform, 1 g of barium hydroxide and 15 mL of saturated barium hydroxide were added. The organic layer was then filtered to a new flask.
25mL of this organic solution was then titrated with the 0.03835M perchloric acid (HCLO4) with a spin bar and 4 drops of crystal violet indicator solution.The yellow endpoint occurred after just 2.499mL of addition of titrant.
From this data, how would I determine the moles of nicotine and grams of nicotine in the original tobacco sample?
Would appreciate any help! Thanks.
It was determined that the calculated molarity of HCLO4 was 0.03835M.
3.0117 grams of tobacco was weighed out and shaken vigorously when 100 mL of 90% toluene-10% chloroform, 1 g of barium hydroxide and 15 mL of saturated barium hydroxide were added. The organic layer was then filtered to a new flask.
25mL of this organic solution was then titrated with the 0.03835M perchloric acid (HCLO4) with a spin bar and 4 drops of crystal violet indicator solution.The yellow endpoint occurred after just 2.499mL of addition of titrant.
From this data, how would I determine the moles of nicotine and grams of nicotine in the original tobacco sample?
Would appreciate any help! Thanks.