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Message: <blockquote data-quote="MikeDemakes" data-source="post: 2369929" data-attributes="member: 817084">Hey, guys. I did a lab last week where we had to determine the nicotine in tobacco and I am having trouble with the calculations.It was determined that the calculated molarity of HCLO4 was 0.03835M.3.0117 grams of tobacco was weighed out and shaken vigorously when 100 mL of 90% toluene-10% chloroform, 1 g of barium hydroxide and 15 mL of saturated barium hydroxide were added. The organic layer was then filtered to a new flask.25mL of this organic solution was then titrated with the 0.03835M perchloric acid (HCLO4) with a spin bar and 4 drops of crystal violet indicator solution.The yellow endpoint occurred after just 2.499mL of addition of titrant.From this data, how would I determine the moles of nicotine and grams of nicotine in the original tobacco sample?Would appreciate any help! Thanks.</blockquote>