i'am doing an experiment on "study of effect of antibiotics on microorganism"
and in the experimental procedure it says autoclave PDA medium at 15 pound pressure.
is it really necessary to autoclave?what if i do not autoclave?are there any alternatives?
and i dont have autoclave machine
aim of autoclave as i learnt is to "sterilize" as my aim is to culture microorganism .it will get unsterilized in course of experiment then why should i autoclave?
and in the experimental procedure it says autoclave PDA medium at 15 pound pressure.
is it really necessary to autoclave?what if i do not autoclave?are there any alternatives?
and i dont have autoclave machine
aim of autoclave as i learnt is to "sterilize" as my aim is to culture microorganism .it will get unsterilized in course of experiment then why should i autoclave?