I did a lab aimed to determine the Aspartate Aminotransferase activity in human plasma.
We measured the absorbance of different concentrations of NADH and from this worked out the extinction coefficient of 5.713 (3dp)
We also measured absorbance of a control sample and 2 patient samples. The absorbance was measured at 15 second intervals and a graph was plotted.
I now need to work out enzyme activity in U/L. How do I do this using the information I have collected?? Is there a standard equation I should be using??
We measured the absorbance of different concentrations of NADH and from this worked out the extinction coefficient of 5.713 (3dp)
We also measured absorbance of a control sample and 2 patient samples. The absorbance was measured at 15 second intervals and a graph was plotted.
I now need to work out enzyme activity in U/L. How do I do this using the information I have collected?? Is there a standard equation I should be using??